You can tell it’s turned on because there will be a recording symbol in the status bar, which is red when it’s making snapshots and white when it’s on standby.īecause it stores the snapshots, that means you can take breaks, close the image, turn off the computer, come back a month later. The recorder docker makes a snapshot of the canvas every few seconds, or at the end of every stroke. Because this docker relies on FFMpeg, it cannot be used on Android. The recorder docker simplifies making a time lapse, by taking a snapshot every stroke and then letting you render it to a video file with ffmpeg.

This is called a time lapse! Normally a time lapse is recorded using outside software, like OBS, and then sped up around 16 times, and they are used to convey the whole amount of effort that went into an image. We show here that residues of Numb that are phosphorylated by atypical protein kinase C (aPKC) to mediate apicobasal polarity in epithelial cells are not required for polarization of Numb in T cells, indicating that the role of aPKC is not conserved between lymphocytes and epithelia.You may have seen artists show little progress movies of their work. TACTICS was used to dissect the regulation of polarization of the cell fate determinant, Numb, in migrating lymphocytes. Similar to flow cytometric analysis, subsets of the data can be gated and reanalyzed to explore the relationships between different parameters. Scatter plots representing microscopic parameters or their derivations such as polarity ratios are linked to the original data such that clicking on each dot enables a link to images and movies of the corresponding cell. A key advantage of our analysis platform, 'TACTICS', is the capacity to visualize individual data points in the context of large data sets, similar to standard approaches in flow cytometry.

We describe a new approach for interactive analysis of time-lapse microscopy, and apply this approach to elucidating whether polarity regulation is conserved between epithelial cells and lymphocytes.

Time lapse tool license key